An Atlas of Human Brain Cell Variation - Project Summary/Abstract the Human Brain Exhibits Profound Diversity…

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An Atlas of Human Brain Cell Variation - Project Summary/Abstract the Human Brain Exhibits Profound Diversity in Biological Function and Vulnerability to Disease. Despite the Biomedical and Cultural Importance of Inter-Individual Variation, We Know Relatively Little About Its Underlying Cellular and Molecular Substrates. IN This Work We Will Leverage New Technologies in Single-Cell and Spatial Genomics to Construct an Atlas of Human Brain Cell Variation. We Will Analyze Tens of Millions of Cells from More Than 200 People by Single-Nucleus Rna-Seq and Single-Nucleus Atac-Seq, and a Subset of These by Spatial Transcriptomics. Analysis of These Data Will Seek to Understand: The Static Versus Dynamic Molecular and Spatial Features of Each Cell Type; the Ways in Which Human Genetic Variation Shapes the Molecular Repertoire of Each Cell Type; and the Constellations of Cellular and Molecular Features That CO-Vary, Appearing Together in the Same Brains. We Seek Especially with This Work to Understand the Functional Connections Between These Phenotypes and: (I) the Gene Regulatory Processes That Drive and Shape IT; (II) Genetic Variation Associated with Brain Diseases; and (III) Structural Brain Variation Measured in Hundreds of Neuroimaging Studies. The Project Will Deliver an Essential Data Resource for Cellular, Molecular, Genetic and Translational Neuroscience – While Expanding Our Understanding of the Ways the Human Brain Varies Across Different People.